Abstract

Macrophages are known to release active oxygen species during phagocytosis or in response to triggering with opsonized zymosan or phorbol myristate acetate. Chemiluminescence intensity from such active species is so low that it has been detected previously by utilizing luminol or lucigenin as a photoamplifier. Using a highly sensitive photon counting system developed recently by our group, we detected very weak chemiluminescence from mouse peritoneal macrophages without use of luminol or lucigenin.

© 1987 Optical Society of America