Abstract
We have performed direct experimental comparisons between confocal and nonconfocal microscopes, using an apparatus that functions simultaneously in both modes. A subresolution pinhole illuminated by a focused laser beam is imaged by a microscope objective onto an object, which is mechanically scanned. A portion of the scattered light is captured by the objective, on the long side of which are two pellicle beam splitters. After one is a confocal detection arrangement, consisting of a pinhole followed by a detector. The other beam splitter is followed by a nonconfocal arrangement, an adjustable finite aperture and a detector. The two images are formed simultaneously, with every aspect of the system identical, except for the detector configurations.
© 1989 Optical Society of America
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