Abstract

A laser microbeam system has been build up, which generates a microbeam radiating the surface of cell and having a selfhealing hole on it. Through the hole the microbeam makes, the external flourescent substance and gene material get into its interior. We have successfully achieved an instant expression of Gus gene in tobacco pollen, with transfer rate as high as two percent. As for animal cells, DAPI-DNA transferred into cancer cells in human stomach.

© 1990 Optical Society of America