Abstract

In this paper, we report a fluorescence sensor for detecting glutathione (GSH), FITC which served as fluorescent donors, and gold nanoparticles (AuNPs, quencher)served as fluorescent acceptors. Energy transfer form FITC to AuNPs because of the electrostatic adsorption, resulting in the quenching of FITC fluorescence. After the addition of glutathione, FITC detach from the surface of gold nanoparticles and the fluorescence will restore because of the strong force of Au-S bond. Based on this principle, we construct a new method to detect GSH and give fluorescence tests in different experimental. The results revealed that there is a good linear in the range of 0.9 ~ 256mg / L in detecting the concentration of GSH, and the correlation coefficient is 0.992. We obtain a satisfying result and it can be used to detect the high concentrations of glutathione content.

© 2014 Optical Society of America