Subtraction Imaging Based on Confocal Microscopy with Array Detection

Famin Wang; Famin Wang; Jian Chang; Yunhai Zhang; Xin miao; Hangfeng Li; Hangfeng Li

doi:10.1364/BODA.2021.JTu4A.20

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper JTu4A.20
•https://doi.org/10.1364/BODA.2021.JTu4A.20

Subtraction Imaging Based on Confocal Microscopy with Array Detection

Famin Wang, Jian Chang, Yunhai Zhang, Xin miao, and Hangfeng Li

Optical Molecular Probes, Imaging and Drug Delivery 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Joint Poster Session I (JTu4A)

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F. Wang, J. Chang, Y. Zhang, X. miao, and H. Li, "Subtraction Imaging Based on Confocal Microscopy with Array Detection," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper JTu4A.20.

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Abstract

A method based on image subtraction is provided to improve the three-dimensional resolution and SNR in confocal microscopy with array detection. By applying the method we can enhance lateral resolution ~0.256λ and axial resolution ~0.723λ.

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