Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Quantifying kinetics and dynamics of DNA repair proteins using Raster-scan Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching

Not Accessible

Your library or personal account may give you access


DNA double-strand breaks (DSBs) are one of the most lethal DNA damage occurs in mammalian cells. Improper repair of DSBs may leads to mutations, chromosomal translocations, apoptosis, genetic instability [1, 2], increases the chance to cancer development [3] and immune deficiency [4]. In this study the confocal Raster scan Image Correlation Spectroscopy technique is used to study kinetics and dynamics of double stand break repair proteins after γ-irradiation of mammalian cells. Diffusion and binding constants were obtained by fitting with different physical models. Results were compared to ones obtained by creating high density DNA damage with a laser and subsequently performing Fluorescence Recovery after Photobleaching over the damage area. This work presents similarities and differences in double strand break repair response between γ-irradiation versus laser damage.

© 2011 Optical Society of America

PDF Article
More Like This
Image-based Fluorescence Recovery After Photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms

S. Daddi Oubekka, R. Briandet, F. Waharte, M.-P. Fontaine-Aupart, and K. Steenkeste
80871I European Conference on Biomedical Optics (ECBO) 2011

DNA Repair Protein Dynamics through Single-Molecule Fluorescence

Keith R. Weninger, Lauryn E. Sass, Vanessa C. DeRocco, Trevor Anderson, and Dorothy A. Erie
LSThD3 Laser Science (LS) 2009

Evaluation of LED therapy at 945 nm on bone repair by micro x-ray fluorescence spectroscopy and scanning electron microscopy

Alexandre G. Diamantino, Renata A. Nicolau, Marco A. de Oliveira, and Ana Maria E. Santo
80921S European Conference on Biomedical Optics (ECBO) 2011

Select as filters

Select Topics Cancel
© Copyright 2023 | Optica Publishing Group. All Rights Reserved