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Quantitative Multi-plexed Fluorescence Spectroscopy in the Presence of a Strong Fluorescent Marker

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Abstract

Localized reflectance spectra containing fluorescence emissions from a strong marker (fluorescein) are accurately modeled. The model is used to estimate metrics of vascular physiology and correct PpIX for absorption and scattering-based distortions.

© 2016 Optical Society of America

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