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Light fluence estimation by imaging photoswitchable probes with temporally unmixed multispectral optoacoustic tomography

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We report on a method based on the temporal domain properties of reversibly switchable fluorescent proteins (RSFPs) in combination with real-time acquisition of volumetric multi-spectral optoacoustic data in order to map light fluence distribution deep in a scattering medium. The new approach has the particular advantage of being independent from the high variability of fluence patterns in different tissues as well as wavelength-dependent light attenuation.

© 2016 Optical Society of America

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