Abstract

High performance liquid chromatography (HPLC) has several advantages over gas chromatography (GC). For example, HPLC enables thermally unstable and nonvolatile compounds to be analyzed. However, a detector for HPLC has not fully been advanced. Recently, mass spectrometry and Fourier transform infrared spectrometry, coupled with HPLC, have been used for qualitative analysis, whereas UV absorption spectrometry has been widely used for trace detection in liquid chromatographic effluents. Recently, the use of laser excitation in fluorescence analysis has opened up new possibilities for ultra sensitive detection. However, the rejection of scattered excitation light is still a tedious problem. In the present paper, laser-induced photoacoustic spectroscopy (LIPAS) is applied to monitoring of HPLC effluents. We have designed a flow cell for coupling LIPAS with HPLC and have tested detection limit, stability, reproducibility, and linearity of the LIPAS detector.

© 1981 Optical Society of America