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Conferences > PCTA > 1988 > PCS > PCS159

Fluorescence Photobleaching Recovery Measurements of Facilitated Diffusion

E. R. Pike, H. J. Gould, and F. Watanabe

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Abstract

The feasibility of a direct measurement of sliding of proteins along a DNA chain by the fluorescence photobleaching recovery technique is demonstrated. The results obtained with a protein, histone H1, provides a firm basis for the understanding of the "facilitated diffusion" mechanism by which site-specific DNA binding proteins are thought to find efficiently their unique loci.

© 1988 Optical Society of America

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