Abstract

I will present our recent work on femtosecond laser manipulation based on digital micromirror devices (DMD) and binary holography; and its applications in developing the next-generation two-photon excitation (TPE) microscopes. The DMD-based beam shaper can arbitrarily control the wavefront of a femtosecond laser at a speed of up to 32 kHz. When integrated with a custom-built laser scanning microscope, new functions such as multi-depth imaging, arbitrary surface imaging can be realized. Further application of the Lee holography leads to a new form of scanless TPE microscope that performs XYZ random-access scanning based on a single DMD with a work volume of ~ 100 × 200 × 500 µm³; selective optical stimulation can be achieved by simply extending the dwell time of the laser focal point. The new DMD TPE microscope may find important applications in brain research, realizing random-access imaging and optical stimulation with tens of microsecond temporal resolution.

© 2017 Optical Society of America