Abstract
We present the first study of the hot vibrational population in a photoexcited heme protein directly observed in the picosecond anti-Stokes Raman spectrum.(1) Our pump-probe Raman experiment with 8ps time resolution unambiguously detects the excited vibrational levels of deoxyhemoglobin (deoxyHb) which are populated following optical excitation. Assuming thermal distribution of vibrational energy, we estimate a vibrational temperature of 36 K above room temperature within our 8 ps resolution. The 1/e time constant for vibrational cooling is 2 to 5ps, after deconvolving the laser pulsewidth.
© 1991 Optical Society of America
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