Abstract
We monitored the fluorescence emission rate of single dye molecules on a dry surface with the near-field scanning optical microscope (NSOM).1 A Texas Red fluorophore was covalently attached to a short strand of a DNA molecule and bound to an amino-propyl-silanized glass coverslip.2 After imaging, the NSOM probe was stationed over a single molecule and its emission rate was recorded as function of time until its photodestruction. Counts were integrated for 5-20 ms and typical count rates were 5-30 kHz. A typical emission time trace is shown in Fig. 1.
© 1996 Optical Society of America
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