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Optica Publishing Group
  • Quantum Electronics and Laser Science Conference
  • OSA Technical Digest (Optica Publishing Group, 2002),
  • paper QPD13

Photon correlation spectroscopy of Fluorescence Resonance Energy Transfer

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We report the use of a Hanbury-Brown Twiss apparatus to measure auto- and cross-correlations from pairs of dye molecules tethered to single strands of DNA and coupled by Fluorescence Resonance Energy Transfer (FRET). We observe non-classical photon antibunching, and we note that certain features of the observed correlations most likely arise from conformational fluctuations in the biomolecular substrate. These techniques should provide experimental access to molecular dynamics at nanosecond (and longer) timescales.

© 2002 Optical Society of America

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