Abstract

We have measured the temperature-annealing-cycling hole spectra of 5-(and 6-) carboxy-X-rhodamine N-hydroxy succinimidyl ester (RhSE) and the RhSE molecule with binding to the oligonucleotide of ten bases of Guanine (RhSE-10G) doped in polyvinyl alcohol (PVOH) films, respectively. Figure 1 shows the temperature-annealing-cycling hole spectra of zero phonon hole (ZPH) of RhSE/PVOH and RhSE-10G/PVOH, which were taken after burning 5 and 10 mins with a flux of ~ 40 mW/cm² at λ_B ~ 5812 Å, respectively. It is found that the holes are reduced by 40 % and 50 % in the annealing spectra at T_a ~ 18 K. However, part of the holes are recovered when the systems are cycled back to the burning temperature at T_c = 6 K. It is found that the hole reduction in the annealing spectrum of RhSE-10G/PVOH is more dominant than that of RhSE/PVOH.

© 1994 Optical Society of America