Abstract
In the past few years, microscope tomography has been intensively studied.1-5 Agard and Sedat restored the 3-D structure of biological cells from the focus series images given by a conventional epi-fluorescence microscope, by inverting the 3-D optical transfer function of the system.1 However, since the 3-D OTF of an epi-fluorescence microscope is angularly band-limited,6 the 3-D spatial frequency components only within the angular band can be restored in their method. As a result, the longitudinal resolution in the restored 3-D structure is not at all satisfactory.
© 1989 Optical Society of America
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