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  • Frontiers in Optics 2009/Laser Science XXV/Fall 2009 OSA Optics & Photonics Technical Digest
  • OSA Technical Digest (CD) (Optica Publishing Group, 2009),
  • paper STuB3
  • https://doi.org/10.1364/SRS.2009.STuB3

Resolution Enhancement and Classification of Virus Particles in Cellular Tomography*

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Abstract

The tomographic reconstruction from whole-cell electron tomography, which is used in the study of viruses in situ, is generally noisy and geometrically distorted due to low electron dose and incomplete projection data. Identical copies of virus particles are expected to be present in a cell, thus by combining data from different virus particles at the same lifecycle stage, a higher resolution structure can be obtained. This classification and data fusion problem is solved using a maximum likelihood estimation approach via an expectation-maximization algorithm. The classification result agrees with the visual impression from the tomographic data and the final reconstruction of each class agrees with the reference structure from single particle cryo-EM analysis.

© 2009 Optical Society of America

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