Abstract
We have employed multicolor single molecule imaging techniques for probing the assembly of cytokine receptor complexes in the plasma membrane of living cells. Posttranslational labeling techniques were employed for labeling the receptor subunits of a cytokine receptor in living cells with quantum dots and suitable organic dyes. Receptor organization in submicroscopic structures at the plasma membrane was unveiled by triple-color super-resolution imaging. Based on single molecule tracking, the diffusion and interaction dynamics of individual signaling complexes were unraveled.
© 2012 Optical Society of America
PDF ArticleMore Like This
James H. Werner, M. Lisa Phipps, Peter M. Goodwin, Patrick J. Cutler, Diane S. Lidke, and Bridget S. Wilson
LWL3 Laser Science (LS) 2010
Bassam Hajj, Jiji Chen, Sara Abrahamsson, Mats Gustaffson, and Maxime Dahan
FM3D.1 Frontiers in Optics (FiO) 2012
Rainer Pepperkok
TuC1 Laser Applications to Chemical and Environmental Analysis (LACSEA) 2004