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Aptamer and DNA hybridization assays on gold fiber optic sensors with nanoparticle signal enhancement

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Abstract

Detection of allergens and micro bacteria on a fiber optic SPR sensor is performed by means of an oligonucleotide bio-recognition surface. In this work, the DNA surface is optimized by mixing with PEG monolayers to suppress unspecific and enhance specific detection. Furthermore, due to the surface characteristic of DNA functionalized gold nanoparticles the bioassay sensitivity is increased and a mean of real-time multiplexing is introduced.

© 2014 Optical Society of America

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