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Stepwise Optical Saturation Microscopy: Obtaining Super-Resolution Images with Conventional Fluorescence Microscopes

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Abstract

We propose stepwise optical saturation (SOS) microscopy, in which two conventional fluorescence images are linearly combined to extend the resolution by 41%. We experimentally perform the SOS microscopy with one-photon (confocal) and multiphoton excitation fluorescence.

© 2018 The Author(s)

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