Abstract

Two-photon excited fluorescence microscopy [1] is emerging as a possible alternative of confocal microscopy. Using two-photon excitation a three-dimensional resolution is obtained as a direct result of the tight focusing of the ultrashort excitation pulses. Consequently, it can be performed in a conventional fluorescence microscope. In the present study, two-photon excited fluorescence microscopy is performed for different types of samples and combined with time- and wavelength resolved spectroscopy [2, 3].

© 1996 Optical Society of America