Abstract

The optical response of wild-type green-fluorescent protein (GFP) has been measured at room temperature following 40-fs, 400-nm photoexcitation. In the spectral range of the stationary fluorescence of GFP, delayed emission due to excited-state proton transfer (ESPT) can be induced. The entire spectro-temporal response is simulated quantitatively using a novel dynamical model which includes an energy-dependent rate constant for ESPT, intra- and intermolecular flow of vibrational energy, and an additional non-radiative decay pathway leading to internal conversion.

© 2002 Optical Society of America