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  • The Thirteenth International Conference on Ultrafast Phenomena
  • 2002 OSA Technical Digest Series (Optica Publishing Group, 2002),
  • paper MC5
  • https://doi.org/10.1364/UP.2002.MC5

Ultrafast elementary events in the excited state of wild-type green-fluorescent protein

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The optical response of wild-type green-fluorescent protein (GFP) has been measured at room temperature following 40-fs, 400-nm photoexcitation. In the spectral range of the stationary fluorescence of GFP, delayed emission due to excited-state proton transfer (ESPT) can be induced. The entire spectro-temporal response is simulated quantitatively using a novel dynamical model which includes an energy-dependent rate constant for ESPT, intra- and intermolecular flow of vibrational energy, and an additional non-radiative decay pathway leading to internal conversion.

© 2002 Optical Society of America

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