Abstract
We applied oblique-incidence reflectivity difference microscopes (a form of polarization-modulated nulling ellipsometry) to detection of biomolecular microarrays without external labeling in a study of protein reactions with surface-immobilized targets. We show that the optical reflectivity difference signals can be quantitatively related to changes in surface mass density of molecular layers as a result of the reactions. Our experimental results demonstrate the feasibility of using oblique-incidence reflectivity difference microscopes for high-throughput proteomics research such as screening unlabeled protein probes against libraries of surface-immobilized small molecules.
© 2008 Optical Society of America
Full Article | PDF ArticleMore Like This
Xiangdong Zhu, James P. Landry, Yung-Shin Sun, Jeff P. Gregg, Kit S. Lam, and Xiaowen Guo
Appl. Opt. 46(10) 1890-1895 (2007)
J. P. Landry, X. D. Zhu, and J. P. Gregg
Opt. Lett. 29(6) 581-583 (2004)
Chenggang Zhu, Ru Chen, Yuzhangyang Zhu, Xu Wang, Xiangdong Zhu, Lan Mi, Fengyun Zheng, and Yiyan Fei
Appl. Opt. 55(33) 9459-9466 (2016)