Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence

Not Accessible

Your library or personal account may give you access

Abstract

A laser flow cytometer based on scanning flow cytometry has been assembled. The unpolarized and linearly polarized light-scattering profiles, as well as the side emitted light in different spectral bands, were measured, allowing the simultaneous and real-time determination of the effective size and the effective refractive index of each spherelike particle. Additionally, each particle could be identified from depolarization and fluorescence measured simultaneously. The tests with aqueous samples of polystyrene spheres, fluorescent or nonfluorescent, and phytoplankton cells demonstrate that the system is able to retrieve size and refractive index with an accuracy of 1% and that the depolarization and fluorescence measurements allow the classification of particles otherwise indistinguishable.

© 2008 Optical Society of America

Full Article  |  PDF Article
More Like This
Size and refractive index of individual marine particulates: a flow cytometric approach

Steven G. Ackleson and Richard W. Spinrad
Appl. Opt. 27(7) 1270-1277 (1988)

Flow cytometric determination of size and complex refractive index for marine particles: comparison with independent and bulk estimates

Rebecca E. Green, Heidi M. Sosik, Robert J. Olson, and Michele D. DuRand
Appl. Opt. 42(3) 526-541 (2003)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (3)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (8)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved