Abstract
Spectral domain phase microscopy (SDPM) has been reported in the literature as a functional extension to low-coherence interferometry, which enables nanoscale measurement of a scatter’s displacement. The signal in SDPM is generated from structural images that lack molecular specificity. This paper investigates the expansion of phase analysis to fluorescence self-interference signals to provide functional information about a sample. Spectral domain fluorescence coherence phase microscopy is demonstrated for nanoscale resolution motion detection of fluorescent particles with a signal-to-noise ratio limited resolution of . This paper demonstrates the feasibility of combining phase processing with fluorescence self-interference, which may be useful for future applications such as cell rheology.
© 2011 Optical Society of America
Full Article | PDF ArticleMore Like This
Michael A. Choma, Audrey K. Ellerbee, Changhuei Yang, Tony L. Creazzo, and Joseph A. Izatt
Opt. Lett. 30(10) 1162-1164 (2005)
Audrey K. Ellerbee, Tony L. Creazzo, and Joseph A. Izatt
Opt. Express 15(13) 8115-8124 (2007)
Hansford C. Hendargo, Mingtao Zhao, Neal Shepherd, and Joseph A. Izatt
Opt. Express 17(7) 5039-5051 (2009)