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Weak two-photon absorption applied to the rapid prototyping of cell scaffolds

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Abstract

Two-photon polymerization (TPP) has been employed to generate deep structures using the biocompatible and optically transparent monomer ethoxylated bisphenol A dimethacrylate (EO=6) (EBPADMA) and 4, 4-Bis(diethylamino)benzophenon as the photoinitiator. The two-photon absorption cross section of the initiator was measured to be 1 GM (1GM=1×1050cm4sphoton1) in EBPADMA. Here we have explored a weak absorption regime whereby deep structures (300μm) can be generated in a single pass. This allows rapid fabrication of structures suitable for cell scaffolds where the length scales are small, 10μm, but are required over long ranges, cm. The dependence of the TPP properties on the writing power, speed, exposure time and NA, of the focusing lens were studied in detail. Diffraction calculations for the focusing optics employed show that spherical aberration plays a significant role in determining the feature sizes achieved.

© 2016 Optical Society of America

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