Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Time-domain measurements reveal spatial aberrations in a sub-surface two-photon microscope

Abstract

We show that in a nonlinear microscopy system the effects of chromatic and spherical aberrations are revealed by a difference in the focal positions corresponding to the shortest pulse duration and the minimum lateral resolution. By interpreting experimental results from a high-numerical-aperture two-photon microscope using a previously reported spatio-temporal model, we conclude that the two-photon autocorrelation of the pulses at the focal plane can be used to minimize both the chromatic and spherical aberrations of the system. Based on these results, a possible optimization strategy is proposed whereby the objective lens is first adjusted for minimum autocorrelation duration, and then the wavefront before the objective is modified to maximize the autocorrelation intensity.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
Spherical aberration measurement of a microscope objective by use of calibrated spherical particles

Dahi Ghareab Abdelsalam and Michel Stanislas
Appl. Opt. 56(16) 4766-4771 (2017)

Exploiting chromatic aberration for image-based microscope autofocus

Derek N. Fuller, Albert L. Kellner, and Jeffrey H. Price
Appl. Opt. 50(25) 4967-4976 (2011)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved