Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Ray tracing matrix approach for refractive index mismatch aberrations in confocal microscopy

Not Accessible

Your library or personal account may give you access

Abstract

The 2×2 ray tracing matrix (RTM) method is employed for the description of optical aberrations caused by the refractive index mismatch (RIM) in fluorescent confocal polarization microscopy. We predict and experimentally confirm that due to the RIM a liquid crystal layer with highly non-uniform director distribution appears to be imaged as a layer with non-uniform thickness, which shows up in the roughness of the rear surface. For the off-axial focusing of the probing beam in a droplet dispersed in an immiscible liquid, we have developed an extended method still keeping the 2×2 dimensionality of the RTM.

© 2017 Optical Society of America

Full Article  |  PDF Article
More Like This
Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy

Alberto Diaspro, Federico Federici, and Mauro Robello
Appl. Opt. 41(4) 685-690 (2002)

Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens

Cees J. de Grauw, Jurrien M. Vroom, Hans T. M van der Voort, and Hans C. Gerritsen
Appl. Opt. 38(28) 5995-6003 (1999)

Influence of aberrations on confocal-based remote refractive index measurements

Helen D. Ford, Daniel Francis, Jonathan M. Hallam, and Ralph P. Tatam
Appl. Opt. 58(24) 6474-6485 (2019)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (38)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved