Abstract

Fast, label-free optical identification and quantification of biomolecules and other relevant biological materials in microfluidic devices and the vascular system will play a major role in liquid biopsy and related diagnoses. An optical microscope probing simultaneously non-linear coherent anti-Stokes Raman scattering (CARS) and linear scattering (LS) was used to probe microparticles in aqueous solutions flowed unconstrained in microfluidic channels. Despite the optical complexity of these systems, where out-of-focus microparticles randomly impede CARS and LS, and where water CARS generates a substantial background, we demonstrate that in-focus microparticles can be individually and unambiguously detected when CARS and LS are co-analyzed. The ability to chemically discriminate microscale features in optically realistic flows supports the relevance of multimodal CARS platforms for liquid biopsy.

© 2018 Optical Society of America

Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry

Charles H. Camp Jr., Siva Yegnanarayanan, Ali A. Eftekhar, Hamsa Sridhar, and Ali Adibi
Opt. Express 17(25) 22879-22889 (2009)

Single laser source for multimodal coherent anti-Stokes Raman scattering microscopy

Adrian F. Pegoraro, Aaron D. Slepkov, Andrew Ridsdale, John Paul Pezacki, and Albert Stolow
Appl. Opt. 49(25) F10-F17 (2010)

Coherent anti-Stokes Raman scattering under frequency comb excitation

Alexey Shchekin, Sergey Koptyaev, Maxim Ryabko, Anton Medvedev, Alexey Lantsov, Hong-Seok Lee, and Young-Geun Roh
Appl. Opt. 57(4) 632-638 (2018)

Label-free enzymatic reaction monitoring in water-in-oil microdroplets using ultra-broadband multiplex coherent anti-Stokes Raman scattering spectroscopy

Ryo Imai and Hideaki Kano
Biomed. Opt. Express 13(3) 1506-1515 (2022)

Orientation dependent coherent anti-Stokes Raman scattering of cylindrical microparticle with focused lasers

C. H. Raymond Ooi, W.-L. Ho, and P. Seow
J. Opt. Soc. Am. B 30(9) 2427-2435 (2013)