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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 62,
  • Issue 7,
  • pp. 727-732
  • (2008)

Characterization of the Ag Mediated Surface-Enhanced Raman Spectroscopy of Saxitoxin

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Abstract

The rapid detection and quantification of saxitoxin (STX) is reported using surface-enhanced Raman spectroscopy (SERS) with a colloidal hydrosol of silver nanoparticles. Under the conditions of our experiments, the limit of detection (<i>L</i><sub>D</sub>) for STX using SERS is 3 nM, with a limit of quantification (<i>L</i><sub>Q</sub>) of 20 nM. It is shown that the SERS method is rapid, with spectra being collected in as little as 5 seconds total integration time for a 40 nM STX sample. In order to improve the signal-to-noise ratio, SERS spectra were generally collected with a total integration time of 1 minute (6 accumulations of 10 seconds each), with no need for extensive sample work-up or substrate preparation. Based on these results, the SERS technique shows great promise for the future detection and quantification of STX molecules in aqueous solutions.

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