Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 64,
  • Issue 4,
  • pp. 391-395
  • (2010)

Erythrocyte Protoporphyrin Fluorescence as a Potential Marker of Diabetes

Not Accessible

Your library or personal account may give you access

Abstract

Protoporphyrin (PpIX), a porphyrin derivative, is the intermediate metabolic precursor of the heme molecule. Abnormal metabolism of total erythrocyte PpIX has been observed in diseases such as cancer, lead poisoning, psoriasis, iron deficiency anemia and acute porphyries. Diabetes mellitus (DM) is a complex metabolic syndrome in which hyperglycemia is the primary clinical manifestation and contributes to the diabetic complications. The aim of this study was to evaluate the utility of fluorescence spectroscopy of erythrocyte PpIX for monitoring the early stages of diabetes. A total of 14 male C 57BL mice, 6 weeks old, were divided into two groups: diabetic and non-diabetic. Diabetes was induced by intraperitoneal injection of streptozotocin (SZT). Blood cells were cultured with standard and 50 mM supplemented RPMI medium. Blood smears were prepared and stained for qualitative morphology analysis under optical microscopy. Blood porphyrin autofluorescence was analyzed by fluorescence spectroscopy. Characteristic PpIX emission spectra were obtained by exciting the samples at 405 nm. Average blood glucose was lower in the control group than in the diabetic group (156.50 ± 6 8.11 mg/dL vs. 371.10 ± 6 14.43 mg/dL, P < 0.05). Both diabetic and glucose-cultured erythroblasts showed a significant decrease (around 30.5% and 40%, respectively) in the emission band intensity at 635 nm. Our results indicate that the erythrocyte PpIX profile could be used as a biological monitor for diabetes.

PDF Article
More Like This
Longitudinal monitoring of pancreatic islet damage in streptozotocin-treated mice with optical coherence microscopy

Won Yeong Park, Jaeyoon Kim, Hoan Le, Bumju Kim, Per-Olof Berggren, and Ki Hean Kim
Biomed. Opt. Express 14(1) 54-64 (2023)

Application of fluorescence lifetime imaging microscopy to monitor glucose metabolism in pancreatic islets in vivo

Zhongying Wang, Maani Archang, Tatyana Gurlo, Elaine Wong, Scott E. Fraser, and Peter C. Butler
Biomed. Opt. Express 14(8) 4170-4178 (2023)

Combination of pathological and spectroscopic characterization to promote diagnosis of retinal pigment epithelium-Bruch’s membrane complex in a diabetic rat model

Yang Chen, Ting Xie, Minlu Ye, Qiaoling Lai, Yuting Wang, Yunchao Xu, Wenyi Chen, Weidong Zheng, Shangyuan Feng, and Yan Huang
Biomed. Opt. Express 12(4) 2221-2235 (2021)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved