Abstract
The beamline design, microscope specifications, and initial results from the
new mid-infrared beamline (IRENI) are reviewed. Synchrotron-based spectrochemical
imaging, as recently implemented at the Synchrotron Radiation Center in Stoughton,
Wisconsin, demonstrates the new capability to achieve diffraction limited chemical
imaging across the entire mid-infrared region, simultaneously, with high
signal-to-noise ratio. IRENI extracts a large swath of radiation (320 hor. × 25
vert. mrads<sup>2</sup>) to homogeneously illuminate a commercial infrared (IR)
microscope equipped with an IR focal plane array (FPA) detector. Wide-field images
are collected, in contrast to single-pixel imaging from the confocal geometry with
raster scanning, commonly used at most synchrotron beamlines. IRENI rapidly
generates high quality, high spatial resolution data. The relevant advantages
(spatial oversampling, speed, sensitivity, and signal-to-noise ratio) are discussed
in detail and demonstrated with examples from a variety of disciplines, including
formalin-fixed and flash-frozen tissue samples, live cells, fixed cells, paint
cross-sections, polymer fibers, and novel nanomaterials. The impact of Mie
scattering corrections on this high quality data is shown, and first results with a
grazing angle objective are presented, along with future enhancements and plans for
implementation of similar, small-scale instruments.
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