Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 67,
  • Issue 11,
  • pp. 1270-1274
  • (2013)

A Fluorescent Nanoprobe Based on Graphene Oxide Fluorescence Resonance Energy Transfer for the Rapid Determination of Oncoprotein Vascular Endothelial Growth Factor (VEGF)

Not Accessible

Your library or personal account may give you access

Abstract

Oncoprotein vascular endothelial growth factor (VEGF) is one of the most critical growth factors that regulates tumor growth and division. The vascular endothelial growth factor (VEGF) is also an important biomarker for different diseases and clinical disorders. Herein, we propose a graphene oxide (GO) fluorescence resonance energy transfer (FRET)-based aptasensor for rapid, sensitive, and selective detection of VEGF in homogeneous solution. The fluorescent dye-labeled anti-VEGF aptamer is adsorbed on the surface of GO via π-π interaction between the flat planar GO sheets and the ring structures in the nucleobases, which results in the fluorescence quenching of the dye due to the highly effective FRET from the dye to GO. Upon recognition and binding with the target VEGF, it specifically forms a VEGF/aptamer complex and then release from the GO surface, leading to the restoration of fluorescence signal of the dye. This GO-based sensing platform exhibits high sensitivity and specificity toward VEGF versus other proteins, with the detection limits corresponding to 2.5×10<sup>−10</sup> M. The sensitivity of this new type of aptamer-based assay is at least one order of magnitude higher than that of conventional homogeneous optical assays. Moreover, the application of this nanosensor for human serum sample analysis is also demonstrated. The GO/aptamer-based assay approach holds great promise as a general platform for detection of a variety of target molecules.

PDF Article
More Like This
In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy

Lu Zhang, Yunhe Ding, Xinjian Chen, Dehui Xiang, Fei Shi, Yanyun Chen, Shenshen Yan, Xinyuan Zhang, Jie Tian, Sobha Sivaprasad, Yang Du, Zhi Yang, and Bei Tian
Biomed. Opt. Express 12(11) 7185-7198 (2021)

Near-infrared fluorescence lymphatic imaging in vascular endothelial growth factor-C overexpressing murine melanoma

Sunkuk Kwon, Fred Christian Velasquez, and Eva M. Sevick-Muraca
Biomed. Opt. Express 9(10) 4631-4637 (2018)

Quantum-dot-conjugated graphene oxide as an optical tool for biosensor

Erin Jenrette, Sangram K. Pradhan, Gugu Rutherford, Jasmin Flowers, Duc Ha, and Aswini K. Pradhan
Opt. Express 23(19) 25017-25027 (2015)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved