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Efficient multiplexed illumination and imaging approach for Fourier ptychographic microscopy

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Abstract

A Fourier ptychographic microscope (FPM) can obtain images with high resolution and a wide field of view (FOV). However, the time-consuming process of image acquisition and computation leads to low reconstruction efficiency. Therefore, we propose a state-multiplexed method through an optimized illumination pattern to accelerate FPM. First, to reduce the redundancy of the spectrum and analyze the impact of missing a certain sub-spectrum on overall spectrum reconstruction, we use an image quality evaluation method to obtain the differential expression between missing a certain LED lighting and all LED lighting. Second, we use the difference expression to select the important LEDs and obtain an optimized illumination pattern that lights up only the LEDs in the central area and the edge LEDs. Then, we update the multiplexing method with the new, to the best of our knowledge, illumination pattern and obtain satisfactorily reconstructed images. Finally, we validate the effectiveness and efficiency of our method with both simulation and experiments. Compared with the traditional method, our method accelerates the reconstruction speed of FPM while ensuring a large FOV and high resolution, saving about 73% of time.

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Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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