Abstract

We present our recent progress on the development of high-resolution optical microscopy with the third near infrared window (NIR-Ⅲ) light for deep tissue imaging. Recently, we developed optical coherence microscopy (OCM) and two-photon excited fluorescence microscopy with NIR-Ⅲ light. The developed OCM allows us to perform label-free three-dimensional imaging with the lateral and axial resolution of 3.4 and 3.8 µm in tissue imaging, and mouse brain imaging at a depth of up to 1.8 mm was also demonstrated. By using the developed two-photon excited fluorescence microscope with NIR-Ⅲ light, we demonstrated two-photon imaging with the lateral and axial resolution of 2.4 and 7.7 µm at a depth of 2.2 mm in the mouse brain. Both results show that NIR-Ⅲ light is a promising choice for deep tissue imaging.

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