Abstract
We applied high-temporal-resolution optical imaging utilizing both the fast intrinsic optical signal (fIOS) and voltage-sensitive dyes (VSDs) to observe the spatiotemporal characteristics of rat somatosensory cortex during electrical stimulation. We find that changes in both the fIOS and VSD signals occur rapidly after the stimulus is applied, suggesting that both membrane depolarization and transmembrane ion movement occur shortly after the stimulus, preceding the more gradual physiological changes in oxygen consumption revealed by the slower component of the intrinsic optical signal. We find that the VSD signal spreads through a much larger area of cortex than the fIOS.
© 2008 Optical Society of America
Full Article | PDF ArticleMore Like This
Jonathan A. N. Fisher, Eugene F. Civillico, Diego Contreras, and Arjun G. Yodh
Opt. Lett. 29(1) 71-73 (2004)
Yang-Guo Li, Qiu-Xiang Zhang, Lei Liu, Franklin R. Amthor, and Xin-Cheng Yao
Opt. Express 18(7) 7210-7218 (2010)
Qiu-Xiang Zhang, Rong-Wen Lu, Yang-Guo Li, and Xin-Cheng Yao
Opt. Lett. 36(23) 4692-4694 (2011)