Abstract

We report on the realization of a sensitive microspectroscopy and imaging approach based on a three-color femtosecond coherent anti-Stokes Raman scattering (CARS) technique with high spectral, time, and spatial resolution. Independently tunable, high-repetition rate optical parametric oscillators were used to attain a dynamic range of 5 orders of magnitude for time-domain CARS signal. The attained sensitivity permitted tracing the decay of weak and structurally complex Raman active modes in soft condensed matter. Application of this approach to imaging of the biological specimen shows a great potential in quantitative characterization of live biological media with an ability to access inter- and intra-molecular interactions.

© 2011 Optical Society of America

Three-color multiplex CARS for fast imaging and microspectroscopy in the entire CHn stretching vibrational region

Jae Yong Lee, Se-Hwa Kim, Dae Won Moon, and Eun Seong Lee
Opt. Express 17(25) 22281-22295 (2009)

A multimodal platform for nonlinear optical microscopy and microspectroscopy

Hongtao Chen, Haifeng Wang, Mikhail N. Slipchenko, YooKyung Jung, Yunzhou Shi, Jiabin Zhu, Kimberly K. Buhman, and Ji-Xin Cheng
Opt. Express 17(3) 1282-1290 (2009)

Ionization penalty in nonlinear Raman neuroimaging

Aleksandr A. Voronin, Ilya V. Fedotov, Lyubov V. Doronina-Amitonova, Olga I. Ivashkina, Marina A. Zots, Andrei B. Fedotov, Konstantin V. Anokhin, and Aleksei M. Zheltikov
Opt. Lett. 36(4) 508-510 (2011)

Imaging skeletal muscle using second harmonic generation and coherent anti-Stokes Raman scattering microscopy

Christian P. Pfeffer, Bjorn R. Olsen, Feruz Ganikhanov, and François Légaré
Biomed. Opt. Express 2(5) 1366-1376 (2011)

Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the deep near infrared

Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, and Hiro-o Hamaguchi
Opt. Lett. 33(9) 923-925 (2008)