Refocusing based on amplitude analysis in color digital holographic microscopy

Jérôme Dohet-Eraly; Catherine Yourassowsky; Frank Dubois

doi:10.1364/OL.39.001109

Optics Letters
Vol. 39,
Issue 5,
pp. 1109-1112
(2014)
•https://doi.org/10.1364/OL.39.001109

Refocusing based on amplitude analysis in color digital holographic microscopy

Jérôme Dohet-Eraly, Catherine Yourassowsky, and Frank Dubois

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Jérôme Dohet-Eraly, Catherine Yourassowsky, and Frank Dubois, "Refocusing based on amplitude analysis in color digital holographic microscopy," Opt. Lett. 39, 1109-1112 (2014)

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- Holography
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The topics in this list come from the Optics and Photonics Topics applied to this article.

About this Article
History
- Original Manuscript: December 10, 2013
- Revised Manuscript: January 14, 2014
- Manuscript Accepted: January 14, 2014
- Published: February 17, 2014
Virtual Issues
Virtual Journal for Biomedical Optics Vol. 9, Iss. 5

Abstract

A refocusing criterion adapted to red–green–blue (RGB) digital holographic microscopy is established. It is applicable for both amplitude and phase objects. This color criterion is based on a monochromatic criterion, using the integrated modulus amplitude. Simulated RGB holograms show the value of having color information, even for colorless samples; in addition, the position of the focus plane along the optical axis is determined more accurately. Simulations take into account both the numerical apertures of lenses and noise during the holographic process. We also implement an algorithm exponentially reducing the computation time required for detecting the focus plane. The method is validated on experimental holograms.

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$n$		$M_{d}^{R G B}$	$M_{d}^{R}$	$M_{d}^{G}$	$M_{d}^{B}$
5%	$μ$	49.9	50.1	49.7	49.6
	$σ$	1.07	1.70	1.49	1.55
10%	$μ$	50.0	49.8	49.6	50.2
	$σ$	1.37	1.85	1.80	1.65
20%	$μ$	50.2	50.3	50.4	50.4
	$σ$	2.46	2.70	2.56	2.70

$n$		$M_{d}^{R G B}$	$M_{d}^{R}$	$M_{d}^{G}$	$M_{d}^{B}$
5%	$μ$	50.1	50.1	50.5	50.1
	$σ$	3.10	4.09	3.26	3.71
10%	$μ$	50.1	49.8	50.6	49.9
	$σ$	2.56	4.20	3.07	4.02
20%	$μ$	50.3	50.2	50.6	50.3
	$σ$	3.61	5.21	4.19	4.71

$n$		$M_{d}^{R G B}$	$M_{d}^{R}$	$M_{d}^{G}$	$M_{d}^{B}$
5%	$μ$	49.9	50.1	49.7	49.6
	$σ$	1.07	1.70	1.49	1.55
10%	$μ$	50.0	49.8	49.6	50.2
	$σ$	1.37	1.85	1.80	1.65
20%	$μ$	50.2	50.3	50.4	50.4
	$σ$	2.46	2.70	2.56	2.70

$n$		$M_{d}^{R G B}$	$M_{d}^{R}$	$M_{d}^{G}$	$M_{d}^{B}$
5%	$μ$	50.1	50.1	50.5	50.1
	$σ$	3.10	4.09	3.26	3.71
10%	$μ$	50.1	49.8	50.6	49.9
	$σ$	2.56	4.20	3.07	4.02
20%	$μ$	50.3	50.2	50.6	50.3
	$σ$	3.61	5.21	4.19	4.71

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Equations (6)

Optics Letters