Abstract

The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was $11.2\times 7.0\mathrm{mm}$ ($\text{horizontal}\times \text{vertical}$), and the corresponding pixel resolution was $1.2\times 1.2\mathrm{\mu m}$. OCM images were compared to traditional histology stained with Nissl and Luxol fast blue (LFB). In particular, the orientation of the fibers was analyzed and quantified in wide-field OCM.

© 2015 Optical Society of America

Polarization sensitive optical coherence microscopy for brain imaging

Hui Wang, Taner Akkin, Caroline Magnain, Ruopeng Wang, Jay Dubb, William J Kostis, Mohammad A Yaseen, Avilash Cramer, Sava Sakadžić, and David Boas
Opt. Lett. 41(10) 2213-2216 (2016)

Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography

Shau Poh Chong, Conrad W. Merkle, Dylan F. Cooke, Tingwei Zhang, Harsha Radhakrishnan, Leah Krubitzer, and Vivek J. Srinivasan
Opt. Lett. 40(21) 4911-4914 (2015)

Ex vivo and in vivo imaging of myelin fibers in mouse brain by coherent anti-Stokes Raman scattering microscopy

Yan Fu, T. Brandon Huff, Han-Wei Wang, Haifeng Wang, and Ji-Xin Cheng
Opt. Express 16(24) 19396-19409 (2008)

Optical coherence tractography using intrinsic contrast

Craig J. Goergen, Harsha Radhakrishnan, Sava Sakadžić, Emiri T. Mandeville, Eng H. Lo, David E. Sosnovik, and Vivek J. Srinivasan
Opt. Lett. 37(18) 3882-3884 (2012)

Full-field optical coherence microscopy with optimized ultrahigh spatial resolution

Antoine Federici and Arnaud Dubois
Opt. Lett. 40(22) 5347-5350 (2015)