Abstract

A method for high-resolution imaging that we call virtual fluorescence emission difference microscopy ($v\mathrm{FED}$) is presented. In $v\mathrm{FED}$ the analyzed samples are scanned only by a doughnut-shaped pattern and imaged by a detector array, which is very different from the previous FED system. By using photon reassignment, we can obtain imaging results with matched solid and hollow point spread functions, and the difference between them is used to estimate the spatial distribution of the analyzed sample. This method results in greatly simplified equipment in the configuration and enhanced imaging speed. Results show that the resolution can be enhanced by at least 27% compared with that in confocal microscopy with a point detector, or is 1.8–2-fold higher than that in wide-field microscopy. Plus, negative intensities can be avoided by using $v\mathrm{FED}$ during the subtraction process, leading to the elimination of the deformation in reconstructed images.

© 2015 Optical Society of America

Resolution enhancement of saturated fluorescence emission difference microscopy

Guangyuan Zhao, Cuifang Kuang, Zhihua Ding, and Xu Liu
Opt. Express 24(20) 23596-23609 (2016)

Ratiometric photon reassignment based on fluorescence lifetime to improve resolution in pulse STED microscopy

Shaocong Liu, Zhimin Zhang, Yubing Han, Lu Yang, Cuifang Kuang, and Xu Liu
Opt. Lett. 46(13) 3304-3307 (2021)

Sparse denoising and adaptive estimation enhances the resolution and contrast of fluorescence emission difference microscopy based on an array detector

Sylvain Prigent, Stéphanie Dutertre, Aurélien Bidaud-Meynard, Giulia Bertolin, Grégoire Michaux, and Charles Kervrann
Opt. Lett. 48(2) 498-501 (2023)

Three-dimensional resolution and contrast-enhanced confocal microscopy with array detection

Baoliang Ge, Yifan Wang, Yujia Huang, Cuifang Kuang, Yue Fang, Peng Xiu, Zihao Rong, and Xu Liu
Opt. Lett. 41(9) 2013-2016 (2016)

Eliminating deformations in fluorescence emission difference microscopy

Shangting You, Cuifang Kuang, Zihao Rong, and Xu Liu
Opt. Express 22(21) 26375-26385 (2014)