Abstract

The determination of the mode and rapidity of motion of individual molecules within a biological sample is becoming a more and more common analysis in biophysical investigations. Single molecule tracking (SMT) techniques allow reconstructing the trajectories of individual molecules within a movie, provided that the position from one frame to the other can be correctly linked. The outcomes, however, appear to depend on the specific method used, and most techniques display a limitation to capture fast modes of motion in a crowded environment. We demonstrate here that the limitations encountered by conventional SMT can be significantly overcome by employing alternative approaches based on image spatial-temporal correlations, enabling to visually extract quantitative insights on the ensemble mode of motion of fluorescently labeled biomolecules that would otherwise be inaccessible.

© 2016 Optical Society of America

Quantitative spectroscopy of single molecule interaction times

H.-H. Boltz, A. Sirbu, N. Stelzer, M. J. Lohse, C. Schütte, and P. Annibale
Opt. Lett. 46(7) 1538-1541 (2021)

Mode analysis of higher-order transverse-mode correlation beams in a turbulent atmosphere

H. Avetisyan and C. H. Monken
Opt. Lett. 42(1) 101-104 (2017)

Particle tracking by repetitive phase-shift interferometric super resolution microscopy

Itay Gdor, Xiaolei Wang, Matthew Daddysman, Yuval Yifat, Rosemarie Wilton, Mark Hereld, Marie-Françoise Noirot-Gros, and Norbert F. Scherer
Opt. Lett. 43(12) 2819-2822 (2018)

Characterization of the self-rotational motion of stored red blood cells by using optically-induced electrokinetics

Wenfeng Liang, Yuechao Wang, Hemin Zhang, and Lianqing Liu
Opt. Lett. 41(12) 2763-2766 (2016)

Digital holographic microscopy as a tool to study the thermal shape fluctuations of lipid vesicles

Christophe Minetti, Victoria Vitkova, Frank Dubois, and Isak Bivas
Opt. Lett. 41(8) 1833-1836 (2016)