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Improving the estimation of flow speed for laser speckle imaging with single exposure time

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Abstract

Laser speckle contrast imaging is a full-field imaging technique for measuring blood flow by mapping the speckle contrast with high spatial and temporal resolution. However, the statically scattered light from stationary tissues seriously degrades the accuracy of flow speed estimation. In this Letter, we present a simple calibration approach to calculate the proportions of dynamically scattered light and correct the effect of static scattering with single exposure time. Both the phantom and animal experimental results suggest that this calibration approach has the ability to improve the estimation of the relative blood flow in the presence of static scattering.

© 2016 Optical Society of America

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