Design rules for combined label-free and fluorescence Bloch surface wave biosensors

Francesco Michelotti; Riccardo Rizzo; Alberto Sinibaldi; Peter Munzert; Christoph Wächter; Norbert Danz

doi:10.1364/OL.42.002798

Optics Letters
Vol. 42,
Issue 14,
pp. 2798-2801
(2017)
•https://doi.org/10.1364/OL.42.002798

Design rules for combined label-free and fluorescence Bloch surface wave biosensors

Francesco Michelotti, Riccardo Rizzo, Alberto Sinibaldi, Peter Munzert, Christoph Wächter, and Norbert Danz

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Francesco Michelotti, Riccardo Rizzo, Alberto Sinibaldi, Peter Munzert, Christoph Wächter, and Norbert Danz, "Design rules for combined label-free and fluorescence Bloch surface wave biosensors," Opt. Lett. 42, 2798-2801 (2017)

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- Remote Sensing and Sensors
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About this Article
History
- Original Manuscript: May 10, 2017
- Revised Manuscript: June 16, 2017
- Manuscript Accepted: June 19, 2017
- Published: July 11, 2017
Virtual Issues
Virtual Journal for Biomedical Optics Vol. 12, Iss. 9

Abstract

We report on the fabrication and physical characterization of optical biosensors implementing simultaneous label-free and fluorescence detection and taking advantage of the excitation of Bloch surface waves at a photonic crystal’s truncation interface. Two types of purposely designed one-dimensional photonic crystals on molded organic substrates with micro-optics were fabricated. These crystals feature either high or low finesse of the Bloch surface wave resonances and were tested on the same optical readout system. The experimental results show that designing biochips with a large resonance quality factor does not necessarily lead in the real case to an improvement of the biosensor performance. The conditions for optimal biochip design and operation of the complete bio-sensing platform are established.

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			Periodic 1D-PC		Topping Bi-Layer
Type	Geometry	$N$	$d_{H}$ [nm]	$d_{L}$	$d_{H}^{'}$ [nm]	$d_{L}^{'}$
1	$L {(HL)}^{N} H^{'} L^{'}$	3	120	340	${Ta}_{2} O_{5}$	${SiO}_{2}$
1		3	120	340	20	20
2		2	120	275	${TiO}_{2}$	${SiO}_{2}$
2		2	120	275	20	20

		Theory					Experiment
		$λ_{LF}$				$λ_{EXC}$	$λ_{LF}$
Type	Pol	$W$ [mdeg]	$D$	$S_{LF}$ [deg/RIU]	${FoM}_{LF} [{RIU}^{- 1}]$	FEF	$W$ [mdeg]	$D$	$S_{LF}$ [deg/RIU]	${FoM}_{LF} [{RIU}^{- 1}]$
1	TE	6	0.85	29.4	4165	64	60	0.08	26.7	35.6
1	TM	550	0.01	22.7	0.41	8	NA	NA	21.3	NA
2	TE	140	0.90	35.4	227	16	200	0.55	31.8	87.5
2	TM	2090	0.01	33.7	0.16	4	NA	NA	NA	NA
SPR	TM	After Ref. [5], $λ_{LF} = 804 nm$					1470	0.76	80.1	41.4

			Periodic 1D-PC		Topping Bi-Layer
Type	Geometry	$N$	$d_{H}$ [nm]	$d_{L}$	$d_{H}^{'}$ [nm]	$d_{L}^{'}$
1	$L {(HL)}^{N} H^{'} L^{'}$	3	120	340	${Ta}_{2} O_{5}$	${SiO}_{2}$
1		3	120	340	20	20
2		2	120	275	${TiO}_{2}$	${SiO}_{2}$
2		2	120	275	20	20

		Theory					Experiment
		$λ_{LF}$				$λ_{EXC}$	$λ_{LF}$
Type	Pol	$W$ [mdeg]	$D$	$S_{LF}$ [deg/RIU]	${FoM}_{LF} [{RIU}^{- 1}]$	FEF	$W$ [mdeg]	$D$	$S_{LF}$ [deg/RIU]	${FoM}_{LF} [{RIU}^{- 1}]$
1	TE	6	0.85	29.4	4165	64	60	0.08	26.7	35.6
1	TM	550	0.01	22.7	0.41	8	NA	NA	21.3	NA
2	TE	140	0.90	35.4	227	16	200	0.55	31.8	87.5
2	TM	2090	0.01	33.7	0.16	4	NA	NA	NA	NA
SPR	TM	After Ref. [5], $λ_{LF} = 804 nm$					1470	0.76	80.1	41.4

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Optics Letters