Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Three-photon fluorescence microscopy with an axially elongated Bessel focus

Not Accessible

Your library or personal account may give you access

Abstract

Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in the field of neuroscience, where the ability to image neurons and their networks with high spatiotemporal resolution is essential. Using an axially elongated focus approximating a Bessel beam, in combination with two-photon fluorescence microscopy, has proven successful at such an endeavor. Here, we demonstrate three-photon fluorescence imaging with an axially extended Bessel focus. We use an axicon-based module that allowed for the generation of Bessel foci of varying numerical apertures and axial lengths, and apply this volumetric imaging tool to image mouse brain slices and for in vivo imaging of the mouse brain.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
Rapid volumetric imaging with Bessel-Beam three-photon microscopy

Bingying Chen, Xiaoshuai Huang, Dongzhou Gou, Jianzhi Zeng, Guoqing Chen, Meijun Pang, Yanhui Hu, Zhe Zhao, Yunfeng Zhang, Zhuan Zhou, Haitao Wu, Heping Cheng, Zhigang Zhang, Chris Xu, Yulong Li, Liangyi Chen, and Aimin Wang
Biomed. Opt. Express 9(4) 1992-2000 (2018)

Three-photon light-sheet fluorescence microscopy

Adrià Escobet-Montalbán, Federico M. Gasparoli, Jonathan Nylk, Pengfei Liu, Zhengyi Yang, and Kishan Dholakia
Opt. Lett. 43(21) 5484-5487 (2018)

Volumetric two-photon fluorescence imaging of live neurons using a multimode optical fiber

Raphaël Turcotte, Carla C. Schmidt, Martin J. Booth, and Nigel J. Emptage
Opt. Lett. 45(24) 6599-6602 (2020)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved