Abstract
Optical sectioning with high-throughput, a high signal-to-noise ratio (SNR), and submicrometer resolution is crucial, but challenging, to three-dimensional visualization of large biological tissue samples. Here we propose line-scanning imaging with digital structured modulation for optical sectioning. Our method generates images with a significantly improved SNR, compared to wide-field structured illumination microscopy (WF-SIM), without residual modulation artifacts. We image a ${14.5}\;{\rm mm} \times {11.5}\;{\rm mm}$ horizontal view of mouse brain tissue at a pixel resolution of ${0.32}\;\unicode{x00B5}{\rm m} \times {0.32}\;\unicode{x00B5}{\rm m}$ in 101 s, which, compared to WF-SIM, represents a significant improvement on imaging throughput. These results provide development opportunities for high-throughput, high-resolution large-area optical imaging methods.
© 2021 Optical Society of America
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