Abstract
We propose a novel, to the best of our knowledge, single-shot quantitative phase imaging (QPI) technique with the phase modulation of a liquid crystal spatial light modulator (LC-SLM) under white light illumination. By studying the phase modulation characteristics of an LC-SLM under white light illumination, images captured at different wavelengths are equivalent to those captured at different defocus distances when loading a Fresnel lens pattern on the LC-SLM. Consequently, a color camera is able to simultaneously acquire multi-intensity images at different defocus distances. Finally, the phase is retrieved from a single-shot color image using the transport of intensity equation. To demonstrate the flexibility and accuracy of our method, a photoetched phase object and human red blood cells are quantitatively measured. An investigation of living yeast cells is conducted to verify the dynamic measurement capability. The proposed method provides a simple, efficient, and flexible means to accomplish real-time high-resolution quantitative phase imaging without sacrificing the field of view (FOV), which can be further integrated into a conventional microscope to achieve real-time microscopic QPI.
© 2022 Optica Publishing Group
Full Article | PDF ArticleMore Like This
Dajiang Lu, Qi Xing, Meihua Liao, Guohai Situ, Xiang Peng, and Wenqi He
Opt. Lett. 47(7) 1754-1757 (2022)
Yang Chen, Xuejuan Wu, Linpeng Lu, Jiahao Wei, Yumin Wu, Qian Chen, and Chao Zuo
Opt. Lett. 47(23) 6061-6064 (2022)
Chen Fan, Junxiang Li, Yijun Du, Zirui Hu, Huan Chen, Zewen Yang, Gaopeng Zhang, Lu Zhang, Zixin Zhao, and Hong Zhao
Opt. Express 31(21) 33830-33841 (2023)