Abstract

A system is described and evaluated for the simultaneous measurement of the intrinsic optical properties of tissue: the scattering coefficient, the absorption coefficient, and the anisotropy factor. This system synthesizes the theory of two integrating spheres and an intervening scattering sample with the inverse adding–doubling algorithm, which employs the adding–doubling solution of the radiative transfer equation to determine the optical properties from the measurement of the light flux within each sphere and of the unscattered transmission. The optical properties may be determined simultaneously, which allows for measurements to be made while the sample undergoes heating, chemical change, or some other external stimulus. An experimental validation of the system with tissue phantoms resulted in the determination of the optical properties with a <5% deviation when the optical density was between 1 and 10 and the albedo was between 0.4 and 0.95.

© 1993 Optical Society of America

Optical properties of normal and carcinomatous bronchial tissue

Jianan Qu, Calum MacAulay, Stephen Lam, and Branko Palcic
Appl. Opt. 33(31) 7397-7405 (1994)

Inverse hybrid technique for determining the optical properties of turbid media from integrating-sphere measurements

I. V. Yaroslavsky, A. N. Yaroslavsky, T. Goldbach, and H.-J. Schwarzmaier
Appl. Opt. 35(34) 6797-6809 (1996)

Determining the optical properties of turbid media by using the adding–doubling method

Scott A. Prahl, Martin J. C. van Gemert, and Ashley J. Welch
Appl. Opt. 32(4) 559-568 (1993)

Multiple polynomial regression method for determination of biomedical optical properties from integrating sphere measurements

Jan S. Dam, Torben Dalgaard, Paul Erik Fabricius, and Stefan Andersson-Engels
Appl. Opt. 39(7) 1202-1209 (2000)

Accuracy of retrieving optical properties from liquid tissue phantoms using a single integrating sphere

Vinoin Devpaul Vincely and Karthik Vishwanath
Appl. Opt. 61(2) 375-385 (2022)