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Two-photon fluorescence microscopy with a diode-pumped Cr:LiSAF laser

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Abstract

We demonstrate the acquisition of high-quality two-photon fluorescence microscopy images using an all-solid-state self-mode-locked Cr:LiSAF laser. We contrast the performance of the two-photon technique with single-photon confocal fluorescence microscopy images taken with an argon-ion laser. Examples of improved depth penetration and reduced dye bleaching are presented.

© 1997 Optical Society of America

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