Abstract

An extensive study has been made of the angular dependence of the intensity as well as the quasi-elastic intensity correlation function of light scattered from a dilute suspension of E. Coli K₁₂ bacteria in solution using the photon correlation technique. We have demonstrated that it is essential to consider the shape as well as the internal structure of bacteria in order to account fully for both the scattered intensity and the field self-correlation function as reduced from the intensity correlation function. The simple structure model previously proposed accounts well for both the intensity and diffusion data with a reasonable set of parameters. For the case of motile bacteria the observed field correlation function at small scattering angles is well represented by assuming a mixture of diffusive and straight line swimming motions of the bacteria. At larger scattering angles contributions of rotational motions become significant.

© 1978 Optical Society of America

Rotational–translational models for interpretation of quasi-elastic light scattering spectra of motile bacteria

Michael Holz and Sow-Hsin Chen
Appl. Opt. 17(20) 3197-3204 (1978)

Accuracy of RGD approximation for computing light scattering properties of diffusing and motile bacteria

Michael Kotlarchyk, Sow-Hsin Chen, and Shoji Asano
Appl. Opt. 18(14) 2470-2479 (1979)

Quasi-elastic light scattering from structured particles

S.-H. Chen, M. Holz, and P. Tartaglia
Appl. Opt. 16(1) 187-194 (1977)

Tracking bacterial movements using a one-dimensional fringe system

Michael Holz and Sow-Hsin Chen
Opt. Lett. 2(5) 109-111 (1978)

Tracking E. coli runs and tumbles with scattering solutions and digital holographic microscopy

Anna Wang, Rees F. Garmann, and Vinothan N. Manoharan
Opt. Express 24(21) 23719-23725 (2016)