Abstract

Fluorescence excitation spectra should be corrected for distortions caused by spectral variations in the transmittance/reflectance ratio of the beam splitter and by variations in the transmittance of cell windows. The polarization of the excitation beam may cause a large uncertainty in the beam-splitter correction, but this source of error can be eliminated by using angles of incidence of 15°, or less, at the beam splitter. The small distortions due to the transmittances of the sample-cell and quantum-counter entrance windows can be made to cancel one another it the angle of incidence at the quantum counter is also 15° or smaller.

© 1979 Optical Society of America

Birefringent polarization-independent beam splitter

Alan M. Frank
Appl. Opt. 18(18) 3111-3113 (1979)

Quantum counter photodetectors: a new design

E. D. Cehelnik and K. D. Mielenz
Appl. Opt. 15(9) 2259-2263 (1976)

Saturation in laser excited atomic fluorescence spectrometry: experimental verification

M. B. Blackburn, J.-M. Mermet, G. D. Boutilier, and J. D. Winefordner
Appl. Opt. 18(11) 1804-1807 (1979)

Polarization-sensitive spectroradiometer: two methods for overcoming the problems of measuring reflectance spectra

Siegfried Auer
Appl. Opt. 18(4) 548-552 (1979)

Angular distribution of fluorescence from liquids and monodispersed spheres by evanescent wave excitation

El-Hang Lee, R. E. Benner, J. B. Fenn, and R. K. Chang
Appl. Opt. 18(6) 862-868 (1979)